Experimental results from Lab

Mon_08/13/2012

• The purified digested TOPO clone plasmid DNA has been checked on the agarous gel for purity.
• Digested pAB vector product_081012.tif
• pAB vector has been digested and run on gel. The gel picture is posted on the "Lab" page.
• Purified digested TOPO clone plasmid DNA_NewTaq_081012.tif

Thurs_08/09/2012

• Purified PCR products (new Taq_CloneTech, SuperTaq_Invitrogen) have been cloned into TOPO vector.
• The TOPO clone plasmid DNA has been purified and digested by Ecor1 and Not1.
• The size of digested product has been checked on agarose gel.
• TOPO clone miniprep digested prodcut new taq_080912.tif
• TOPO clone miniprep digested prodcut superTaq_080912.tif
• The results indicated that the SuperTaq has less efficiency.

Thurs_08/02/2012

• The program of PCR reaction using new Taq enzyme (CloneTech_ Advantage HF 2 polymerase) has been modified.
• The PCR reactions using different Taq enzymes have been performed.
• The purity of one set of PCR product was checked on agarous gel.
• 

Above gel showed that New Taq polymerase from cloneTech works for the current PCR reaction conditions + program but not great.

With modifications, the New Taq Polymerase works effectively. The size of PCR product is fell in the range of 600-700 bp as expected.

SuperTaq Plus Polymerase was used to perform the PCR reaction. It works well too (The size of PCR product is fell in the range of 600-700 bp as expected).
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